Evaluation of Hepatoprotective and Antioxidant Properties of litsea Monopetala Methanolic Extract: Insights from FRAP and CCl₄-induced Hepatotoxicity in HEPG2 Cells
DOI:
https://doi.org/10.48047/5wm08130Keywords:
Litsea Polyantha, Total Phenolics, Antioxidant, Hepatoprotective, Carbon Tetrachloride, Biomarkers.Abstract
The hepatoprotective, antioxidative, and bioactive properties of Litsea monopetala methanolic extract (LMME-L) were evaluated in HepG2 cells exposed to CCl₄-induced hepatotoxicity. The study assessed its effects on cell viability, liver enzymes, oxidative stress biomarkers, total phenolic content (TPC), and ferric reducing antioxidant power (FRAP). LMME-L exhibited a concentration
dependent increase in antioxidant activity, as indicated by the FRAP assay, with maximum FeSO₄ equivalent values at higher concentrations. The total phenolic content was determined as 8.6 mg of gallic acid equivalents (GAE) per gram of extract, correlating with its antioxidant properties. In cell based assays, LMME-L improved viability to 97.05 ± 3.16% at 1000 µg/mL, comparable to silymarin. Elevated levels of liver enzymes (AST, ALT, LDH) and oxidative stress markers (MDA) in CCl₄treated cells were significantly reduced by LMME-L, while glutathione (GSH) levels were restored. Combined treatment with LMME-L and silymarin demonstrated synergistic hepatoprotective effects. These results highlight LMME-L’s potential as a natural hepatoprotective agent with strong
antioxidative activity, attributed to its phenolic content. Further investigations into its bioactive compounds and therapeutic mechanisms are recommended.
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