Use of modified gram’s stain in peripheral smear staining - An alternative for Leishman’s stain
DOI:
https://doi.org/10.48047/e0r0rf02Keywords:
Modified gram stain, Leishman stain, Peripheral smear, Blood cells, Morphology, Innovative technique.Abstract
Introduction: Peripheral blood smear is a blood film which has a thin layer of blood smeared on a glass slide which is stained such a way that blood cells can be examined clearly under a microscope. Peripheral blood smear shows the number and shape of the blood cells in which the information derived from the peripheral blood smear has become a cornerstone in Laboratory Hematology and is widely used for screening, case finding, diagnosis, and monitoring of haematological and non-haematological disorders. Examination of stained smears may have certain limitations, such as suboptimal staining and stain precipitate. The diagnostic
relevance of peripheral blood smear has not been lessened by advances in haematology automation and molecular techniques.
Downloads
References
Bessman, D. (1978) ‘Microcytosis caused by RBC fragmentation. Confirmation by RBC size distribution analysis’, JAMA, 239(23), pp. 2475–2476. 2. Bohatirchuk, F.P. (1957) ‘Stain historadiography’, Stain Technol., 32(2), pp. 67–74. 3. Brundha, M.P. and Nivedhita, G. (2020) ‘Analysis of Papanicolaou stain on peripheral smear compared to Leishman’s stain: A prospective study’, International Journal of Clinicopathological Correlation, p. 40. Available at:
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
You are free to:
- Share — copy and redistribute the material in any medium or format for any purpose, even commercially.
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
- The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
- Attribution — You must give appropriate credit , provide a link to the license, and indicate if changes were made . You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
Notices:
You do not have to comply with the license for elements of the material in the public domain or where your use is permitted by an applicable exception or limitation .
No warranties are given. The license may not give you all of the permissions necessary for your intended use. For example, other rights such as publicity, privacy, or moral rights may limit how you use the material.
