Antibacterial Activity of Arginine that Extraction and purification from Staphylococcus aureus
Keywords:
Antibacterial activity, Arginine production, E. coli, Burns, Ninhydrin reagent, Protein.Abstract
Arginine, a semi-essential α-amino acid involved in various metabolic pathways, including protein synthesis, nitric oxide production, and urea cycle function, is conditionally essential in humans, meaning it can become necessary under certain physiological conditions. This study investigated the extraction and purification of L-arginine from bacterial pathogens isolated from clinical samples. Twenty samples were collected from wound and burn infections at hospitals in Baghdad, Iraq. Bacterial isolates were cultured on selective media and identified using a combination of microscopic examination, biochemical tests (including
catalase, coagulase, oxidase, and urease), and the Vitek 2 automated system (bioMérieux).
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