Histological and Immunohistochemical Study of Calcification Leiomyoma uterus in postmenopausal women
Abstract
Calcified fibroids, also known as calcified leiomyomas and myomas, are benign tumors located in the uterus. These tumors have become rigid due to calcium deposits inside the mass of smooth muscles and fibrous connective tissues. Fibroids can undergo
calcification as they mature and begin to deteriorate. This study utilized histology to analyze 20 cases of total hysterectomy or myomectomy and validate their classification as fibroids. We then fixed all these samples in formalin at a 10% concentration. A
histological study has been made by using three types of dyes, hematoxylin and eosin to show the general histological structure, Van Kossa stain to detect the deposition of calcium salts in the tissue, while the immunohistochemical technique has been conducted by using primary and secondary antibodies showing overexpression of osteopontin (OPN) protein within most muscle fibers in fibroids affects the uterine muscle tissue, especially the transversely arranged myofibers compared to the longitudinally arranged myofibers. We found that the overexpression of the osteopontin (OPN) protein in most of the myofibers of the leiomyoma had an effect on the myometrium tissues. Furthermore, there was a notable increase in the expression of OPN in transversally arranged myofibers as compared to longitudinally arranged myofibers.
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References
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-Greg A. Johnson, Robert C. Burghardt, Fuller W. Bazer, Thomas E. Spencer, Osteopontin: Roles in Implantation and Placentation, Biology of Reproduction, Volume 69, Issue 5, 1 November 2003, Pages 1458–1471.
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