MOLECULAR CHARACTERIZATION OF CRISPR- CAS SYSTEM IN CLINICAL STAPHYLOCOCCUS AUREUS ISOLATED FROM IRAQI PATIENTS.
DOI:
https://doi.org/10.48047/vgn67s66Keywords:
S. aureus, molecular characterization, CRISPR-Cas systems, IraqiAbstract
The aim of study was detection the CRISPR-Cas system genes in Staphylococcus aureus in attempt as a new strategy for the control of resistance of S. aureus. Material and methods: The isolation of S. aureus requires, a total of 50 specimens (abscesses, cellulitis, and other forms of soft tissue infection) were collected from Al-Hussein Teaching Hospital in Al-Muthanna governorate, followed by Kirby-Bauer’s Disc, with some tweaks, will be used to determine antibiotic susceptibility. Antibiotics that were utilized were penicillin, azithromycin, Ciprofloxacin, tetracycline, gentamicin, doxycycline, vancomycin, ofloxacin, chloramphenicol, ampicillin, cefoxitin, and oxacillin. Finally Molecular uncovering of crispr-Cas system, The results: Our results revealed that 25 S.aureus isolate obtained from 50 specimen as following 6 (12%)from cellulitis, 7(14%) from abscesses and12 (24%) from soft tissue infections S.aureus isolates showed various response to antibiotics depending on antimicrobial resistance percentages the highest resistance noticed in penicillin(100%) and Ampicillin (100%) followed by Chloramphenicol (80%), Vancomycin(60%), Cefoxitin (60%), Ofloxacin (45%), however the isolates showed more sensitive to following antibiotics in addition All 25 isolates found was not harboured to CRISPER CAS-F1, Furthermore the results reported that all 25 isolates found were not harboured to Cas6 System-II. Conclusion: a high occurrence of multidrug-resistant S. aureus alongside the complete absence of CRISPR-Cas systems, emphasizing the importance of ongoing antimicrobial surveillance and the development of alternative molecular control approaches
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