Comparison between phenotypic and genotypic methods in biofilm formation in MRSA isolates based on zinc oxide quantum dots
DOI:
https://doi.org/10.48047/w5qmnm79Keywords:
MRSA; ZnO-QDs; VITEK2; MDR; XDR; fnbB geneAbstract
Background: The MRSA bacteria are known for their high resistance to many drugs. One of the main virulence factors that support the survival of MRSA and its resistance to most antibiotics is the formation of biofilms.
Objective of study: This study aims to investigate the effect of nanomaterial zinc oxide quantum dots (ZnO-QDs) on resistant bacteria after understanding their antimicrobial resistance patterns, from MRSA to a newer antibiotic, in order to identify any extensively drug-resistant (XDR) isolates..
Materials and Methods: From 100 clinical samples, 20 isolates were identified as MRSA isolates Sensitivity testing was conducted on all isolates using the VITEK2 system. These isolates were classified into three categories: MDR, XDR, and to non-MDR isolates, based on the results of their antibiotic resistance profiles. Biofilm formation ability was investigated using TCPM, TM, CRA tests, and the fnbB gene.
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References
Zong, Z., et al. (2016). Biofilm formation in Staphylococcus aureus and its clinical implications. Clinical Microbiology Reviews,
(2), 437–464.
Hall-Stoodley, L., et al. (2004). Biofilms and chronic infections. Science, 287(5461), 1857-1862.
Stewart, P. S., & Franklin, M. J. (2008). Physiology and functional genomics of Pseudomonas aeruginosa biofilms. Advances in
Microbial Physiology, 54, 227–318.
Stewart, P. S., & Costerton, J. W. (2001). Antibiotic resistance of bacteria in biofilms. The Lancet, 358(9276), 135–138.
Donlan, R. M. (2001). Biofilms and device-associated infections. Emerging Infectious Diseases, 7(2), 277–281.
O’Gara, J. P., & Hickman, M. (2005). Biofilm formation by Staphylococcus aureus: Regulatory and genetic aspects. Journal of
Applied Microbiology, 98(6), 1014–1021.
Geisinger, E., & Adhikari, R. P. (2018). Regulation of biofilm formation in Staphylococcus aureus by the sarA gene. Journal of
Clinical Microbiology, 56(7), e01076-18.
Novick, R. P., et al. (1993). The agr system of Staphylococcus aureus and its role in virulence. Molecular Microbiology, 9(4),
–504.
Fowler, V. G., & Proctor, R. A. (2014). Staphylococcus aureus infections: Clinical manifestations and treatment strategies.
Journal of Infectious Diseases, 209(8), 1083–1091.
Zhang, L., et al. (2014). Antimicrobial properties of zinc oxide nanoparticles and their applications in biofilm inhibition. Journal of
Nanobiotechnology, 12, 39.
Rajendran, R., & Jha, A. K. (2018). Zinc oxide nanoparticles in the biomedical field: Opportunities and challenges. Journal of
Applied Microbiology, 124(6), 1226–1236.
Alam, S. S., et al. (2020). Inhibition of bacterial biofilm formation by zinc oxide nanoparticles. Journal of Nanoparticle Research,
(1), 1–11.
Ghosh, S., et al. (2019). ZnO nanoparticles: Synthesis, antimicrobial and biofilm inhibition potential. Applied Nanoscience, 9(8),
–1203.
Moradi, M., et al. (2016). *Antibacterial and anti-biofilm activity of ZnO nanoparticles against Staphylococcus aureus. Journal of
Biomedical Materials Research, 104(4), 1080–1091.
Li, X., et al. (2013). *Effect of ZnO nanoparticles on biofilm formation in Staphylococcus aureus. Journal of Applied
Microbiology, 114(4), 1044–1054.
Park, J., et al. (2017). Zinc oxide nanoparticles: Antimicrobial and anti-biofilm potential. Journal of Infection and Public Health,
(6), 665–674.
Cramton, S. E., et al. (2001). In vitro studies of biofilm formation by Staphylococcus aureus using the tissue culture plate
method. Journal of Clinical Microbiology, 39(7), 2406–2409.
Fadaei, R., & Hassan, S. S. (2021). Biofilm formation assays: An overview. Current Microbiology, 78, 1–13.
Saha, S., et al. (2021). Molecular mechanisms of biofilm formation in MRSA : Involvement of ica and agr operons. Molecular and
Cellular Biochemistry, 466(1-2), 125–137.
Mikkelsen, H., et al. (2014). Regulation of biofilm formation in Staphylococcus aureus by the ica operon. Journal of
Microbiology, 52(7), 509–518.
Kwiecinski, J., & Peters, G. (2015). Biofilm formation in Staphylococcus aureus and its genetic regulation. Infection and
Immunity, 83(8), 3139–3150.
Desai, M., et al. (2020). Nanoparticle-based biofilm disruption strategies for MRSA infections. Frontiers in Microbiology, 11,
O’Neill, J. (2016). Tackling drug-resistant infections globally: Final report and recommendations. Review on Antimicrobial
Resistance.
Liao, Y., et al. (2017). The application of nanomaterials for biofilm disruption in MRSA infections. Frontiers in Microbiology, 8,
Garza, E. A., & Gonzalez, L. (2021). Potential of zinc oxide nanoparticles in overcoming antimicrobial resistance in biofilms.
International Journal of Nanomedicine, 16, 1569–1582.
Lee, S. H., et al. (2020). ZnO nanoparticles in biofilm disruption and antimicrobial therapy. Therapeutic Advances in Infectious
Disease, 7, 2049936120909824.
Peng, H., et al. (2018). Nanoparticles in antimicrobial applications. Journal of Clinical Microbiology, 56(10), e00979-18.
Nowak, R., et al. (2017). Anti-biofilm activity of nanomaterials and their applications. Current Medicinal Chemistry, 24(21), 2300–
Sirelkhatim, A., et al. (2015). Zinc oxide nanoparticles: A review of antimicrobial properties and mechanisms of action. Frontiers
in Microbiology, 6, 1–10.
Niaz, S., et al. (2019). Nanotechnology in the fight against antimicrobial resistance: A review of antimicrobial nanoparticles for
biofilm disruption. Environmental Toxicology and Pharmacology, 66, 9–17.
Ling TK, Liu Z, Cheng AFJJocm. Evaluation of the VITEK 2 system for rapid direct identification and susceptibility testing of
gram-negative bacilli from positive blood cultures. 2003;41(10):4705-7.
El-Sayed AF, Aboulthana WM, Sherief MA, El-Bassyouni GT, Mousa SMSynthesis, structural, molecular docking, and in vitro
biological activities of Cu-doped ZnO nanomaterials. Scientific reports. 2024.
Ca NX, Hien NT, Fan X, Do PV, Yen VH, Hao PV, Quynh LK, Huong TTT, Quang VXNew insights on the luminescence
properties and Judd-Ofelt analysis of Er-doped ZnO semiconductor quantum dots.RSC advances. 2023.
ABBAS, Haydar Abdulmeer; CHEIKHROUHOU-KOUBAA, Wissem. Influence of Ag2O Nanoparticles on the Ammonia Gas
Sensing Properties of CNT/P3HT Composite Films. Journal of Techniques, 2024, 6.4: 98-106.doi.org/10.51173/jt.v6i4.2577.
ABBAS, Zahraa Neamah, et al. Antibacterial and Antibiofilm Activity of Zinc Oxide Quantum Dots against Methicillin‐resistant
Staphylococcus aureus. Particle & Particle Systems Characterization, 2024, 2400048.
Basnet A, Tamang B, Shrestha MR, Shrestha LB, Rai JR, Maharjan R, Dahal S, Shrestha P, Rai SK Assessment of four in vitro
phenotypic biofilm detection methods in relation to antimicrobial resistance in aerobic clinical bacterial isolates. PloS one. 2023
Abdulqader HA, Abood ZH. Effect of Salicylic Acid on the gene expression of FnbA and FnbB genes in Staphylococcus
hominis. Hum Antibodies. 2024;32(3):139-149. doi: 10.3233/HAB-240023. PMID: 38875028.
Ozturk B, Gunay N, Ertugrul BM, Sakarya S. Effects of vancomycin, daptomycin, and tigecycline on coagulase-negative
staphylococcus biofilm and bacterial viability within biofilm: an in vitro biofilm model. Can J Microbiol. 2016 Sep;62(9):735-43.
doi: 10.1139/cjm-2015-0855. Epub 2016 Apr 19. PMID: 27295353.
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