CREATING A DIET INDUCED OBESITY ZEBRAFISH MODEL FOR ANALYSIS OF METABOLIC PROGRAMMING IN TUMORIGENESIS.
DOI:
https://doi.org/10.48047/xb1szb53Keywords:
Obesity model, tumorigenesis, zebrafish, induced diet, coffee, green tea, overfeedAbstract
BACKGROUND AND AIM: Overweight is now more common than smoking as a modifiable risk
factor for carcinogenesis, accounting for 40% of cancer cases reported in the United States. Using the
framework of the hallmarks of cancer, we here outline how obesity may affect the carcinogenic
hallmarks in somatic cells. To analyze the attenuation of adipocytes in zebrafish by feeding coffee
and green tea infused food. MATERIALS AND METHOD: Zebrafish husbandry involved keeping
the fish at a controlled temperature of 28 degrees Celsius with a light-dark cycle of 14 hours. Feeding
protocol for zebrafish: Four distinct fish groups were examined, each receiving a unique diet. By
providing standard food, the first group (n = 10) was constituted as a control group. By giving an
excess of food to the second group (n = 10). Third group (n=10) was fed food that had been infused
with coffee. Fourth group (n = 10) served meals laced with green tea.
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