A Study of Gene Expression of Inhibin and Activin Subunits (INHA,INHBA) in the Placenta of Women with Missed Miscarriage
DOI:
https://doi.org/10.48047/6f9bwd12Keywords:
.Abstract
Missed Miscarriage (MM) was a special type of spontaneous Miscarriage that the embryo or
fetus has already died but remained in the uterus for days or weeks and with a closed cervical
ostium . Patients might present with or without subtle clinical symptoms such as vaginal bleeding
or abdominal pain. Missed Miscarriage, occuring in approximately 8–20% of clinically confirmed
intrauterine pregnancies, was often confirmed using ultrasonography.
In this study, We used bioinformatics analysis to identify hub genes. Verified the expression
of the genes inhibin subunit alpha(INHA) and inhibin subunit beta A(INHBA ) in women placenta
with MM .Placental tissues were obtained from therapeutic abortion (8-16 weeks, first trimester)
and it was kepped in shield solution at -20 c. Genomic RNA from tissues samples were extracted
by using Direct-zol™ RNA MiniPrep Kit(USA),then INHA and INHBA genes was amplified
through via Reverse Transcription-Pol merase Chain Reaction(RT-PCR).We used The Livak
method, also known as the 2−ΔΔCt method, is a widely used approach for analyzing data from
quantitative real-time PCR (qPCR) experiments to calculate changes in gene expression levels
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