Study the Isolation of mesenchymal stem cells from newly born infants of the fetal-chorionic plate
DOI:
https://doi.org/10.48047/kzjf7p77Keywords:
mesenchymal stem cells, CD105, CD90, CD73, Epidermal growth factor, osteogenic differentiation factors, placenta, collagenase IAbstract
Abstract
Stem cells are exclusive population of cells located in all stages of lifetime that holds capability to
self-renewal plus specialize to group of cell lineages of multiple organs and tissues and considered a
major source of differentiating cell types in the body, stem cells has been considered as part of new
cell therapy of numerous damaged organs for instant embryonic stem cells in term of neurological
cases like stroke and parkinson’s as these cells are transplanted in the body for focal organ damage
repair, differentiation and immunoregulatory response, chemoattractant and stimulate tissue
regeneration Current study detected the presence of mesenchymal stem cells isolated from Iraqi
newborn infants placenta using enzymatic method of collagenase I enzyme and cultured with DMEM
low glucose, through observation of their genetic markers expression such as CD105, CD90 and
CD73 using RT-PCR showed positively folding as well as the onset of their expression levels
reduction as an initiation process for osteoblastic gene expression using epidermal growth factor plus
osteogenic differentiation factors including β-glycerophosphate disodium salt pentahydrate,
Dexamethasone and L-ascorbic acid
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