Evaluating Withaferin A: Neuropsychopharmacological Impact through Pentobarbitone-Induced Sleep, Forced Swim Test, and Spontaneous Locomotor Activity Modulation in Mice

Authors

  • Sujata Wankhede, Nisha Kumari Singh, Parvathi V Hospeti, Amit Mehra, Sonu Dewaji Gathe, Pravin S. Uttekar, A. Tejasri, K. Ramadevi Author

DOI:

https://doi.org/10.48047/gg1ynb35

Keywords:

Withaferin A, Behavioural pharmacology, Neuropsychopharmacology, Pentobarbitone induced sleeping time, Forced Swim Test (FST), Spontaneous Locomotor Activity (SMA), Sedative, Antidepressant-like effects, Withania somnifera

Abstract

Withaferin A, a bioactive compound from Withania somnifera, was evaluated for its neuroprotective and neuropsychopharmacological effects. The study investigated its ability to mitigate oxidative  stress in SH-SY5Y neuronal cells and its impact on sleep, depressive-like behaviour, and locomotion in Swiss albino mice. In the in vitro assay, Withaferin A significantly reduced intracellular reactive oxygen species (ROS) levels induced by hydrogen peroxide in a dose-dependent manner.

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References

Can, A., Dao, D. T., Arad, M., Terrillion, C. E., Piantadosi, S. C., & Gould, T. D. (2012). The mouse forced swim test. JoVE (Journal of Visualized Experiments)(59), e3638.

Chen, L.-X., He, H., & Qiu, F. (2011). Natural withanolides: an overview. Natural product reports, 28(4), 705-740.

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Published

2025-02-03

Issue

Vol. 54 No. 3 (2025): Year 2025, Volume 54, Issue 3

Section

Articles

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How to Cite

Evaluating Withaferin A: Neuropsychopharmacological Impact through Pentobarbitone-Induced Sleep, Forced Swim Test, and Spontaneous Locomotor Activity Modulation in Mice (Sujata Wankhede, Nisha Kumari Singh, Parvathi V Hospeti, Amit Mehra, Sonu Dewaji Gathe, Pravin S. Uttekar, A. Tejasri, K. Ramadevi , Trans.). (2025). Cuestiones De Fisioterapia, 54(3), 1800-1890. https://doi.org/10.48047/gg1ynb35