Precision Unveiled: Robust RP-HPLC Method for Accurate Estimation of Prasugrel, a Platelet Aggregation Inhibitor, in Final Dosage Form
DOI:
https://doi.org/10.48047/CU/54/03/966-978Keywords:
All statistical data confirms the effectiveness of the methods, making them suitable for routine analysis of quality control and stability studies in pharmaceutical dosage form.Abstract
Background: Administered orally, Prasugrel is a third generation thienopyridine that irreversibly inhibits platelet aggregation by binding with P2Y12 receptor. It is available in coated tablets of 5 and 10 mg commercially, but there are very few analytical methods available for estimation of Prasugrel in final dosage form.
Objective: The current study introduces a simple and accurate high-performance liquid chromatographic technique to determine the amount of Prasugrel in the final dosage form Method: The mobile phase consists of Methanol – Buffer (700 + 300, v/v). A column containing octadecylsilane chemically bonded to porous silica particles (Purospher star ® 100 ODS 250 x 4.6 mm, 5 µm) was used as stationary phase. Detection was performed using a variable wavelength ultraviolet-visible detector set at 220 nm for Prasugrel. Solutions were injected into the chromatograph under isocratic condition at a constant flow rate of 1.0 mL/min. The stability
indicating capability of the method was confirmed through a force degradation study.
Downloads
References
Parker WA, Storey RF. The role of platelet P2Y12 receptors in inflammation. British Journal of Pharmacology. 2023.
Furman D, Campisi J, Verdin E, Carrera-Bastos P, Targ S, Franceschi C, et al. Chronic inflammation in the etiology of disease across the life span. Nature medicine. 2019;25(12):1822-32.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
You are free to:
- Share — copy and redistribute the material in any medium or format for any purpose, even commercially.
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
- The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
- Attribution — You must give appropriate credit , provide a link to the license, and indicate if changes were made . You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
Notices:
You do not have to comply with the license for elements of the material in the public domain or where your use is permitted by an applicable exception or limitation .
No warranties are given. The license may not give you all of the permissions necessary for your intended use. For example, other rights such as publicity, privacy, or moral rights may limit how you use the material.
