Green synthesized Silver Nanoparticles using leaf extract of Nicotiana rustica Natural Pesticides against Indian white termites, Odontotermes obesus
DOI:
https://doi.org/10.48047/vv6gqb18Keywords:
Silver Nanoparticles, Plant extract of Nicotiana rustica, Termites, Digital Calorimeter, FTIR, and SEM.Abstract
– Green nanotechnology has widespread applications in various biomedical science fields. In this research paper, green-synthesized silver Nanoparticles, prepared by using Nicotiana rustica extract, were characterized using digital calorimeter, scanning electron microscopy and FTIR. This study thoroughly investigated the insecticidal efficacy of medicinal plants, Nicotiana rustica. Different extraction methods were used and their efficacy with and without nanoparticles was compared. FT-IR analysis indicated the involvement of carboxyl (-C=O), hydroxyl (-OH), C-N stretching of aromatic amines or NO2 symmetric stretching and amine (-NH) functional groups of tobacco leaf extract in the preparation of Silver Nanoparticles. SEM analysis showed that AgNPs is widely distributed in some areas and has irregular and circular shapes that have different diameters with average diameters of, for example, 54.79 nm and 66.05 nm. The focus of the study was on investigating various parameters such as mortality rates, insect survival rates, adult emergence and development stages after treatment periods of 24, 42 and 72 hours. The termite repellent efficacy was significantly higher in treatments with nanoparticles, but the differences were not significant (p>0.05).
Downloads
References
Abd-Elnaby H.M., Abo-Elala G.M., Abdel-raouf U.M & Hamed M.M. (2016). Antibacterial and anticancer activity of extracellular synthesizd silver nanoparticles from marine Streptomyces rochei MHM13. Egypt J. Aquat Res., 42(30), 301-312.
Ahmad F., Fouad H., Liang S.Y., Hu Y. & Mo J.C. (2021). Termites and Chinese agricultural system: Applications and advance in integrated termite management and chemical control. Insect Sci., 28, 2-20.
Azhari Y.S., QX J. & Xia W.S. (2014). Physicochemical properties and chemical composition of Seinat (Cucumis melo var. tibish) seed oil and its antioxidant activity. Grasas Y. Aceites, 65(1), 1-9.
Bayda S., Adeel M., Tuccinardi T., Cordani M. & Rizzolio F. (2019). The history of Nano science and nanotechnology: from chemical-physical applications to Nano medicine. Molecules. 25(1), 112.
Devi G.D., Murugan K. & Selvam C.P. (2014). Green synthesis of silver nanoparticles using Euphorbia hitra (Euphorbiaceae) leaf extract against crop pest of cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae). J. Biopestic. 7, 54.
Gour A. & Jain N.K. (2019). Advances in green synthesis of nanoparticles. Artif. Cells Nanomed. Biotechnol., 47, 844-851.
Govindarajan M., Sivakumar R., Rajeswari M., Yogalakshmi K. (2012). Chemical composition and larvicidal activity of essential oil from Mentha spicata (Linn.) against three mosquito species. Parasital. Res., 110, 2023-2032.
Goyorushko S. (2019). Economic and ecological importance of termites: A global review. Entomol. Sci., 22, 21-35.
Higgins M. (2010, Nov 4). Is Nicotine A Useful Pesticide? Stanford Chemicals. Retrieved 2014, Sep 2024.
Hussain I., Singh N.B., Singh A., Singh H. & Singh S.C. (2016). Green synthesis of nanoparticles and its potential application. Biotechnol. Lett., 38, 545-560.
Jain A.S., Pawar P.S. Sarkar A., Junnuthula V. & Dyawanapelly S. (2021). Bionanofactories for green synthesis of silver nanoparticles: Toward antimicrobial appliactions. Int. J. Mol. Sci., 22(21), 11993.
Karnnai R. & Chowdhary A. (2013). Biosynthesis of silver nanoparticles by eco-friendly method. Indian J. Nanosci., 1, 25-31.
Kumar S. P., Pathak D., Patel A., Dalwadi P., Prasad R., Patel P. & Selvaraj K. (2011). Biogenic synthesis of Silver Nanoparticles using Nicotiana tabaccum leaf extract and study of their antibacterial effect. African Journal of Biotechnology. 10(41), 8122-8130.
Laszlo C., Kaminski K., Guan H., Fatarova M., wei J., Bergounioux A., Schlage W.K., Schorderet-Weber S., Guy P. A., Ivanov N.V., Lamottke K., & Hoeng J. (2022). Fractionation and extraction optimization of potentially valuable compounds and their profiling in six varieties of two Nicotiana species. Molecules, 27(22), 8105.
Paul K. K., Gnanajobitha G., Vanaja M., Rajesh K. S., Malarkodi C. & Pandian K. (2014). Piper nigrum leaf and stem assisted green synthesis of silver nanoparticles and evaluation of its antibacterial activity against agricultural pants pathogens. Sci. World J., (1), 829894.
Ranjith M., Vinod D., Bajya D.R., Manoharan T & Gajalakshmi M (2017). Evaluation of termiticidal activity and phytochemical analysis of Crotalaria burhia (Buch-Ham) and Anacardium Ociidentale (L.). Journal of Pharmacognosy and photochemistry. 6(2), 172-176.
Rautela A., Rani J. & Debnath M. (2019). Green synthesis of silver nanoparticles from Tectona grandis seeds extract: characterization and mechanism of antimicrobial action on different microorganisms. Journal of Analytical Science and Technology, 10, 5.
Regnault-Roger C., Philogene B.J.R. & Vincent C. (Eds). (2005). Biopesticides of plant origin, Lavoisier. 313.
Rehman H.U., Majeed B., Farooqi M.A., Rasul A., Sagheer M., Ali Q. & Akhtar Z.R. (2021). Green synthesis of silver nitrate nanoparticles from Camelina sativa (L.) and its effect to control insect pests of stored grains. Int. J. Trop. Insect Sci., 41, 1-9.
Sahu M.K., Yadav R. & Tiwari S.P. (2023). Recent advances in nanotechnology. Int J Nanomater Nanotechnol Nanomed., 9(1), 15-23.
Sarkar R., Kumbhakar P. & Mitra A.K. (2010). Green synthesis of silver nanoparticles by using onion (Allium cepa) extract and their antibacterial activity. Digest J. Nanomater. Biostructures, 5, 427-432.
Singh P. & Mijakovic I. (2022). Strong antimicrobial activity of silver nanoparticles obtained by the green synthesis in viridibacillus sp. extracts. Front Microbial., 13, 820048.
Stanfill S.B., Oliveira da Silva A.L., Lisko J.G., Lawler T.S., Kuklenyik P., Tyx R.E., Peuchen E.H., Richter P. & Watson C.H. (2015). Comprehensive chemical characterization of Rape tobacco products: Nicotine, un-ionized nicotine, tobacco-specific N’-nitrosamines, polycyclic aromatic hydrocarbons and flavor constituents. Food and Chemical Toxicology, 82, 50-58.
Su N-Y. (2002). Novel technologies for subterranean termite control. Sociobiology, 40, 95-102.
Suman T.Y., Rajasree S.R.R., Kanchana A. & Beena Elizabeth S. (2013). Biosynthesis, characterization and cytotoxic effect of plant mediated silver nanoparticles using Morinda citrifolia root extract. Colloids surf B: Biointerfaces., 106, 74-78.
Tan S. (n.d.). What to know about natural pesticides. webMD. Retrieved May 1, 2024, from https://www.webmd.com/ .
Verma M., Sharma S & Prasad R. (2009). Biological alternatives for termite control: A review. Int. Biodeterior. Biodegrad., 63, 959-972.
Yang X., Han H., Li B., Zhang D., Zhang Z. & Xie Y. (2021) Fumigant toxicity and physiological effects of spearmint (Mentha spicata, Lamiaceae) essential oil and its major constituents against Reticulitermes dabieshanensis. Ind. Crop. Prod., 171, 113894.
Ying S., Guan Z., Ofoegbu P.C., Clubb P., Rico C., He F. & Hong J. (2022). Green synthesis of Nanoparticles: Current Developments and Limitations. Environ. Technol. Innov., 26, 102336.
Zahoor M., Nazir N., Iftikhar M., Naz S., Zekker I. & Burlakovs J. (2021). A review on Silver Nanoparticles: Classification, various methods of synthesis, and their potential roles in biomedical applications and water treatment. Water. 13(16), 2216.
Downloads
Published
Issue
Section
License

This work is licensed under a Creative Commons Attribution 4.0 International License.
You are free to:
- Share — copy and redistribute the material in any medium or format for any purpose, even commercially.
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
- The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
- Attribution — You must give appropriate credit , provide a link to the license, and indicate if changes were made . You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
Notices:
You do not have to comply with the license for elements of the material in the public domain or where your use is permitted by an applicable exception or limitation .
No warranties are given. The license may not give you all of the permissions necessary for your intended use. For example, other rights such as publicity, privacy, or moral rights may limit how you use the material.