Identification of leaf-cutting bees (Megachile sp.) from Northern Sulawesi of Indonesia: study of morphology, morphometric and genetic characteristics
DOI:
https://doi.org/10.48047/8x84md68Keywords:
COI, Leaf-cutting bees, Northern Sulawesi, Phylogenetic, SequencingAbstract
This study aims to identify of leaf-cutting bee (Megachile sp.) in Northern Sulawesi, Indonesia, based on morphology, morphometrics, and genetic characteristics. This study was done from September 2022 to March 2023. Species identification of the bees was carried out at the Museum Zoologicum Bogoriense (MZB). Morphometric measurements were used 25 characters of the body and then analysed using ANOVA. Molecular analysis used the partial Mitochondrial DNA COI sequence. Extract DNA was done from the thorax and amplified by primers LCO 1490 and HCO 2198. Data analysis and the phylogenetic tree were reconstructed using MEGA 11. The results showed that based on morphological characteristics, this species resembles to Megachile atrata Smith., but is not identical based on the wing colours and fine blackish-brown hairs on the abdomen. Hence, we use the term Megachile sp., hereinafter, for this bee. Morphometric analysis showed that 7 characters have significant differences between the populations. Molecular analysis showed that this bee had a low level of species similarity with other Megachile, ranging from 86.47% to 89.39%, even though the closed genetic distance was M. takaoensis (OR231111.1), and the farthest was M. opacifrons (OR796342.1). This study has contributed to the DNA barcoding of this bee in the gene bank database for further research in the future. Further research on this bee is required for conservation and management purposes because this bee is an effective pollinator for crops
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References
Agnarrsson, I & Kuntner, M. 2007. Taxonomy in a changing world: seeking solutions for
a science in crisis. Systematic Biology, 56(3): 531-539.
Anaktototy, Y., Priawandiputra, W., Sayusti, T., Lamerkabel, J.S.A. & Raffiudin, R.
Morphology and morphometric variation of stingless bees in the Moluccas, Indonesia.
Indonesian Journal of Entomology 18(1):10-22. doi: 10.5994/jei.18.1.10
Ascher, J.S. & Pickering 2024. Discover life bee species guide and world checklist, viewed
June 2024, from https://www.discoverlife.org.
Ascher, J.S., Risch, S., Soh, Z.W.W., Lee, J.X.Q., & Soh, E.J.Y. 2016. Megachile leafcutter and resin bees of Singapore (Hymenoptera: Apoidea: Megachilidae). Raffles Bulletin
of Zoology, 32, pp. 33–55, viewed 21 May 2024, from https://lkcnhm.nus.edu.sg/wpcontent/uploads/sites/10/app/uploads/2017/06/S32rbz033-055.pdf.
Bzdyk, E. 2012. A revision of the Megachile subgenus Litomegachile Mitchell with an
illustrated key and description of a new species (Hymenoptera, Megachilidae,
Megachilini). Zookeys, 221, pp. 31–61. doi: 10.3897/zookeys.221.3234
Corlett, R.T. 2004. Flower visitors and pollination in the Oriental (Indomalayan) Region.
Biological Reviews, 79(3), pp. 497–532. doi: 10.1017/S1464793103006341.
Dayrat, B. 2005. Towards integrative taxonomy. Biological Journal of the Linnean Society,
(3): 407-415.
Folmer, O., Black, M., Hoeh, W., Lutz, R., dan Vrijenhoek, R. 1994. DNA primers for
amplification of mitochondrial Cytochrome C oxidase subunit I from diverse metazoan
invertebrates. Molecular Marine Biology and Biotechnology 3(5): 294–299.
Gonzalez, V.H., Engel, M. S. & Hinojosa-Díaz, I.A. 2010. A new species of Megachile
from Pakistan, with taxonomic notes on the subgenus Eutricharaea (Hymenoptera:
Megachilidae). Journal Kansas Entomology Society, 83(1), pp. 58–67, 2010, viewed 24
September 2024, from
https://www.academia.edu/2189208/A_new_species_of_Megachile_from_Pakistan_with
_taxonomic_notes_on_the_subgenus_Eutricharaea_Hymenoptera_Megachilidae).
Gonzalez, V.H., Gustafson, G.T., & Engel, M.S. 2019. Morphological phylogeny of
Megachilini and the evolution of leaf-cutter behavior in bees (Hymenoptera:
Megachilidae). Journal of Melittology 85: 1–123.
Horne, M. 1995. Leaf area and toughness: effects on nesting material preferences of
Megachile rotundata (Hymenoptera: Megachilidae). Annals of Entomological Society
America, 88(6), pp. 868–875. doi:10.1093/aesa/88.6.868
Kim, J.Y. 1997. Female size and fitness in the leaf-cutter bee Megachile apicalis.
Ecological Entomology. 22(3), pp. 275-282. doi:10.1046/j.1365-2311.1997.00062.x.
Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions
through comparative studies of nucleotide sequences, J Mol Evol, 16(2), pp. 111–120. doi:
1007/BF01731581.
Laltanpuii N, Kumar, S., & Mathai, T. 2014. Molecular and phylogenetic analysis of the
genus Orthetrum (Odonata:Anisoptera: Libelluidae) using mitochondrial COI gene.
Science Vision, 14(3): 152-157.
Michener, C.D., & Ivany, J.J.H.S. 1960. Observations on the biology of a leaf cutter bee
"Megachile frontalis" in New Guinea. Papua New Guinea Agricultural Journal, 13(1), pp.
-35.
Michener, C.D. 2007. The Bees of The World, 2nd ed. Baltimore, Maryland: The Johns
Hopkins University Press.
Momose, K., & Karim, A.A.H. 2005. The Plant-Pollinator Community in a Lowland
Dipterocarp Forest. In Pollination Ecology and the Rain Forest. New York: SpringerVerlag, ch. 6, pp. 65–72. doi: 10.1007/0-387-27161-9_6.
Morris, P.J., 2024. Museum of Comparative Zoology, Harvard University. Version 162.
Museum of Comparative Zoology, Harvard University, viewed 17 November 2024,
from https://www.gbif.org/occurrence/4130904379.
Novita., Saepudin, R., & Sutriyono. Morphometrics Analyses of Apis cerana Workers
Cultivated at Different Altitude. Jurnal Sain Peternakan Indonesia. 8(1): 41-56.
Odum, E.P. 1996. Ecological Basics. Translated by Tjahjono Samingan. Gadjah Mada
University Press. Yogyakarta.
Schmitt, T & Haubrich, K. 2008. The genetic structure of the mountain forest butterfly
Erebia euryaleunravels the late Pleistocene and postglacial history of the mountain
coniferous forest biome in Europe. Molecullar Ecology, 17(9): 2194-2207.
Sheffield, C.S., Ratti, C., Packer, L., & Griswold, T. 2011. Leafcutter and mason bees of
the genus Megachile Latreille (Hymenoptera: Megachilidae) in Canada and Alaska.
Canadian Journal of Arthropod Identification 18: 1-107.
Sinu, P.A., & Bronstein, J.L. 2018. Foraging preferences of leafcutter bees in three
contrasting geographical zones. Divers Distrib, 24(5), pp. 621–628, doi:
1111/ddi.12709.
Smith, F. 1853. Catalogue of Hymenopterous Insects in the Collection of the British
Museum. Part 1. Andrenidae and Apidae. Reed Lion Court, Fleet Street: Taylor and
Francis, 1853, viewed 24 September 2024, from
https://www.zportman.com/uploads/8/3/5/4/83540892/catalogue_of_hymenopterous_inse
cts_in_the_british_museum.pdf
Soh, E.J.Y. 2014. Diversity and Trap Nesting Studies of Singaporean Megachile Bees to
Inform Monitoring and Management of Tropical Pollinators. National University of
Singapore, Bachelor Thesis, Singapore, 110 pp.
Soh, E.J.Y., Soh, Z.W.W., Ascher, J. S., & Tan, H.T.W. 2019. Diversity of plants with
leaves cut by bees of the genus Megachile in Singapore. Nature in Singapore, 12, pp. 63–
, viewed 22 May 2024, from https://lkcnhm.nus.edu.sg/wpcontent/uploads/sites/10/app/uploads/2019/11/NIS-2019-0007.pdf
Tallei, T.E., Koneri, R. & Kolondam, B.J. 2017. Sequence Analysis of the Cytochrome C
Oxidase Subunit I Gene of Pseudagrion pilidorsum (Odonata: Coenagrionidae). Makara
Journal Science, 21(212), pp. 43-52. doi: 10.7454/mss.v21i1.7536.
Tamura, K., Stecher, G., & Kumar, S. 2021. MEGA11: Molecular Evolutionary Genetics
Analysis Version 11. Mol Biol Evol, 38(7), pp. 3022-3027, doi: 10.1093/molbev/msab120.
Wilson, J.J., Sing, K., & Sofian-azirun, M. 2013. Building a DNA bercode reference library
for the true butterflies (Lepidoptera) of Peninsula Malaysia: what about the sub species?.
Plos One, 8(11):1-10.
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