Molecular Identification of Pathogenic Bacteria in Sarcophaga peregrina Larvae as a Cause of Ophthalmomyiasis
DOI:
https://doi.org/10.48047/kndes039Keywords:
Ophthalmomyiasis, Sarcophaga peregrina, Pathogenic Bacteria, Molecular IdentificationAbstract
Ophthalmomyiasis, a rare but severe parasitic infestation of the eye, is commonly associated with fly
larvae, particularly Sarcophaga peregrina. This study aims to identify pathogenic bacteria present in S.
peregrina larvae, which may exacerbate infections in ophthalmomyiasis cases. Samples were collected
from high-risk areas in Bitung and Manado, Indonesia, where fly populations thrive in human and
livestock environments. Molecular identification of the larvae was conducted using COI gene sequencing,
while bacterial identification employed 16S rRNA analysis. The results confirmed S. peregrina as the
primary fly species responsible for ophthalmomyiasis. Bacterial analysis revealed the presence of
Stenotrophomonas maltophilia, Bacillus subtilis, Proteus mirabilis, and Enterobacter hormaechei, all of
which are known to contribute to secondary infections and antimicrobial resistance.
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