UV-Visible spectrophotometric method development and validation for the estimation of glipizide in bulk and pharmaceutical formulation
DOI:
https://doi.org/10.48047/6tnh7m30Keywords:
UV-visible spectrophotometer, glipizide, ICH guidelinesAbstract
The primary objective of the present research is to develop a simple, rapid, accurate, reproducible, and economical UV spectrophotometric method for measuring the quantity of glipizide (GLP) in pharmaceutical dosage form and bulk. Using a UV-visible double beam spectrophotometer, the amount of GLP was calculated at an absorption maximum of 273 nm using methanol as solvent. According to the specifications of the ICH guideline, the developed technique was validated for linearity, accuracy, precision, ruggedness, robustness, LOD, and LOQ. With a linear equation of y=0.0172x-0.0141 and a correlation coefficient of 0.9997, the linearity was determined to be 1060 µg/ml. The accuracy was between 98% and 100.50%. The RSD percentages among samples
was 0.19926 and 0.21806 for intra-day and inter-day precision, respectively. The limit of detection (LOD) and limit of quantification (LOQ)were discovered to be 4.77µg/ml and 14.47 µg/ml, respectively. According to ICH Q2(R1) requirements, the developed approach was validated. The innovative approach can be used to analyze drugs in their pharmaceutical dose form.
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References
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