DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF CENTRAL MUSCLE RELAXANT & ANALGESIC DRUG IN PHARMACEUTICAL FORMULATION
DOI:
https://doi.org/10.48047/29rtzs92Keywords:
HPLC, Diclofenac Sodium, Tolperisone Hydrochloride, Method Development, ValidationAbstract
A robust and accurate RP-HPLC method was developed and validated for the simultaneous
estimation of Diclofenac Sodium (DIC) and Tolperisone Hydrochloride (TOL) in pharmaceutical
formulations. The study also employed derivative UV spectrophotometry as a complementary
analytical technique. The UV spectrophotometric method utilized first-order derivative spectra
with zero crossing points at 248 nm for DIC and 226 nm for TOL, ensuring accurate
quantification. The method demonstrated good linearity for DIC (2-10 µg/mL) and TOL (5-25
µg/mL), with correlation coefficients close to 1. Precision, accuracy, and sensitivity were
confirmed through recovery studies, with mean recoveries of 99.087-100.35% and 99.93-
100.46% for DIC and TOL, respectively.
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