ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF FIMASARTAN POTASSIUM TRIHYDRATE AND CILNIDIPINE IN SYNTHETIC MIXTURE BY HPLC METHOD FOR THE TREATMENT OF HYPERTENSION
DOI:
https://doi.org/10.48047/g42py576Keywords:
HPLC, Cilnidipine, Fimasartan Potassium Trihydrate, Method Development, ValidationAbstract
This study aimed to develop and validate an analytical method for the simultaneous
estimation of Fimasartan Potassium Trihydrate and Cilnidipine in a synthetic mixture using
the HPLC method for the treatment of hypertension. A specific, accurate, precise, robust, and
cost-effective HPLC method was developed and validated for the quantitative analysis of
Fimasartan Potassium Trihydrate and Cilnidipine in a fixed-dose combination. The isocratic
elution was performed using a Kromasil C18 column (250 mm × 4.6 mm ID, 5 μm) at 40°C.
The mobile phase consisted of Methanol: 0.1% OPA in water (80:20% v/v) at a flow rate of
1.0 mL/min. The injection volume was 20 μL, and UV detection was carried out at 223 nm
with a total run time of 12 minutes. Fimasartan Potassium Trihydrate and Cilnidipine were
eluted at retention times of 3.57 min and 7.99 min, respectively.
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Copyright (c) 2024 Kunal Borse, Chetan Darade, Chandrashekhar Patil, Prabha Bhong, Deepak Somavanshi, Kajal Pansare, Ganesh Sonawane (Author)
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