Quantitative estimation of ursolic acid by HPTLC in methanolic extract of leaves Streblus asper and Melissa officinalis
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https://doi.org/10.48047/p2zqgm38Keywords:
.Abstract
Chromatographic techniques (HPTLC) was used for qualitative and quantitative determination of ursolic acid in methanolic leaves extract of Streblus asper and Melissa officinalis from the Moraceae and Lamiaceae family. It is considered to contain ursolic acid, confirmed by TLC and by qualitative test. Thus it was quantified using HPTLC a sensitive method for development of marker compounds. The method was carried out on precoated TLC aluminum plates with silica gel F 254 as stationary phase using solvent system as toluene: ethyl acetate: glacial acetic acid (7:3:0.2) in both extract. with Rf value of 0.636. Quantitative analysis was carried out in the absorbance at 530 nm. A good linear relationship (r2 = 0.9939 respective to peak area and height, Respectively) was observed between the concentration ranges of 200ng/spot to 1000ng/spot Thus HPTLC method provides a faster and cost effective quality control for the routine analysis of ursolic acid in extracts containing Streblus asper and Melissa officinalis
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References
Sivamaruthi BS, Prasanth MI, Kesika P, Tencomnao T, Chaiyasut C. (2022) Functional properties of Streblus asper Lour.: a review. Food Science and Technology. 13;42:e11342. 2. Ibrahim NM, Mat I, Lim V, Ahmad R. (2013) Antioxidant activity and phenolic content of Streblus
asper leaves from various drying methods. Antioxidants;2(3):156-66.
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