VALIDATION AND EVALUATION OF AN HPLC METHOD FOR QUANTIFICATION OF VITAMIN D3 IN PHARMACEUTICAL FORMULATIONS

Authors

  • Jaymala Arun Kumawat Assistant Professor, Vishwakarma University, School of Pharmacy, Kondwa, Pune, Maharashtra. 411048 Author
  • Vivek Kumar Associate Professor, LR Institute of Pharmacy, Jabli-Kyar, Solan, Himachal Pradesh. 173223 Author
  • Preeti Research Scholar, Gautam College of Pharmacy, Hamirpur Himachal Pradesh. 177001 Author
  • Landge Dhananjay Ashok Associate Professor, Adsul College of pharmacy, Chas, Ahmednagar, Maharashtra. 414005 Author
  • Uma Shanker Maurya Assistant Professor, Goel Institute of Pharmacy and Sciences, Lucknow, Uttar Pradesh. 226028 Author
  • Pradeep Kumar L Associate Professor, Department of Pharmaceutical Chemistry, Sri K V College of Pharmacy. Author
  • Suryanarayan Dash Assistant Professor, Mayurbhanj Medical Academy, Indapahi, Laxmiposi, Mayurbhanj, Odisha.757107 Author
  • Sharmila Mondal Assistant Professor, Department of Pharmaceutical Technology, JIS UNIVERSITY, 81,Nilgunj Rd, Agarpara, Kolkata, west Bengal. 700109 Author

DOI:

https://doi.org/10.48047/wy5sw824

Keywords:

LINEARITY, ACCURACY, PRECISION, VITAMIN-D3, HPLC,LOD

Abstract

In accordance with the guidelines provided by ICH Q2 (R1), the analytical procedure that you have described for quantifying vitamin D-3 through the use of HPLC is quite comprehensive and has been thoroughly validated. The apparatus appears to be reliable, as it consists of a C-18 column, a mobile phase consisting of methanol and water, and detection at 264 nm, which is an adequate wavelength for vitamin D-3 identification

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References

Siddiqui M.R., AlOthman Z.A., Rahman N. Analytical techniques in pharmaceutical analysis: A review. Arab. J. Chem. 2017;10:S1409–S1421. doi:10.1016/j.arabjc.2013.04.016.2. Taraji M., Haddad P.R., Amos R.I.J., Talebi M., Szucs R., Dolan J.W., Pohl C.A. Rapid Method Development in Hydrophilic Interaction Liquid Chromatography for Pharmaceutical Analysis Using a Combination of Quantitative Structure–Retention Relationships and Design of Experiments. Anal. Chem. 2017;89:1870–1878. doi:10.1021/acs.analchem.6b04282.

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Published

2025-02-20

Issue

Vol. 54 No. 4 (2025): Year 2025, Volume 54, Issue 4

Section

Articles

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How to Cite

VALIDATION AND EVALUATION OF AN HPLC METHOD FOR QUANTIFICATION OF VITAMIN D3 IN PHARMACEUTICAL FORMULATIONS (J. . Arun Kumawat, V. Kumar, Preeti, L. . Dhananjay Ashok, U. . Shanker Maurya, P. Kumar L, S. . Dash, & S. . Mondal , Trans.). (2025). Cuestiones De Fisioterapia, 54(4), 919-927. https://doi.org/10.48047/wy5sw824