Validated reversed-phase liquid chromatographic method with gradient elution for simultaneous determination of the antiviral agents
DOI:
https://doi.org/10.48047/s0w4xs96Keywords:
Sofosbuvir, Daclatasvir; RP- HPLC; Validation, Chromatography.Abstract
A Validated, reversed-phase liquid chromatography (RP-HPLC) method for with gradient elution for simultaneous determination of Sofosbuvir and Daclatasvir in their dosage forms. An accurate, reproducible method was developed and validated. The mobile phase contained a mixture of 90% methanol: 10% water (0.05% OPA). UV detection was performed at a temperature of 30° C., a flow rate of 0.7 ml/min and at a wavelength of 275 nm.
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Ansari Yaasir Ahmed, Singh Sumer, Quazi Majaz, Ahmed Jameel, Ansari Mohd Razi. Stability Indicating HPLC Method Development and Validation for Simultaneous Estimations of Atenolol and Nifedipine in Bulk and Tablet Dosage Form. Journal of Emerging Technologies and Innovative Research. 2019; 6(2): 29-36.
Shaikh Siraj N, Vasave Magan G, Khan Gulam Javed, Patel M Siddik N, Ansari Yasir A Razzaq, Siddiqi Hifzurrahman Md A, Molvi Khurshid I, Chaudhary Jayesh C. Development and Validation of RP-HPLC Method for Estimation of Ondansetron HCL in Bulk and Pharmaceutical Dosage Form. International Journal of Pharmaceutical Research. 2021; 13(2): 1538-1548.
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