PCR test in detecting dermatophyte species among a sample of tinea patients attending to Imamein Kadhimein Medical City
DOI:
https://doi.org/10.48047/3prkkn28Keywords:
Dermatophytosis , Conventional PCR, Trichophyton Mentagrophytes.Abstract
Background: Dermatophytosis is a common infection caused by superficial filament fungi that affects the skin, nails, and hair. It holds global importance, especially for countries located in tropical and subtropical regions.
Objective : identify of dermatophytes species by Conventional PCR method using ITS1 and ITS4 primer.
Methods: cross-sectional study conducted at the dermatology clinic at Imamein Kadhimein Medical City in Baghdad between 22th /January /2024 to 29 /July/2024, the specimens were obtained from all participants, and a potassium hydroxide (KOH) test, cultured on Sabouraud's dextrose agar and polymerase chain reaction (PCR) analysis was utilized to for identification and identification of the dermatophyte species.
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References
Zareshahrabadi, Z., Totonchi, Alireza, Et Al., Molecular Identification And Antifungal Susceptibility Among Clinical Isolates Of Dermatophytes In Shiraz, Iran (2017‐2019). Mycoses, 2021. 64(4): P. 385-393.
Kraševec, N., The Multifaceted Role Of Mating Type Of The Fungus And Sex Of The Host In Studies Of Fungal Infections In Humans. Journal Of Fungi, 2022. 8(5): P. 461.
Iorio, R., Et Al., Dermatophytoses In Cats And Humans In Central Italy: Epidemiological Aspects. Mycoses, 2007. 50(6): P. 491-5.
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